分离的人胎盘合体滋养层细胞膜的Fcγ受体活性

Fc gamma-receptor activity of isolated human placental syncytiotrophoblast plasma membrane.

作者信息

Brown P J, Johnson P M

出版信息

Immunology. 1981 Feb;42(2):313-9.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1458069/

Abstract

Fc gamma-receptor activity of isolated human placental syncytiotrophoblast microvillous plasma membrane (StMPM) vesicle preparations has been determined in an immunoradiometric assay using Sepharose-immobilized protein A to separate free 125I-labelled human IgG from membrane-bound 125I-IgG. This receptor assay has been optimalized in terms of buffer pH and molarity, and used to demonstrate that prior 60 min washing of isolated membranes in 3 M KCl to remove extrinsic membrane-bound protein substantially increases the membrane-binding capacity for IgG. Inhibition studies have determined the syncytiotrophoblast Fc gamma-receptor equilibrium constant for association (Ka) as 4.0 x 10(7) M-1 at 37 degrees and the number of available Fc gamma-receptor sites as 1.5 x 10(14) per mg membrane protein.

摘要

在一项免疫放射分析中，利用固定在琼脂糖上的蛋白A将游离的125I标记的人IgG与膜结合的125I-IgG分离，测定了分离出的人胎盘合体滋养层微绒毛质膜（StMPM）囊泡制剂的Fcγ受体活性。该受体分析在缓冲液pH值和摩尔浓度方面已得到优化，并用于证明在3M KCl中对分离出的膜进行60分钟的预洗涤以去除外在膜结合蛋白，可显著提高膜对IgG的结合能力。抑制研究确定了合体滋养层Fcγ受体的缔合平衡常数（Ka）在37℃时为4.0×10⁷M⁻¹，每毫克膜蛋白上可利用的Fcγ受体位点数量为1.5×10¹⁴个。

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Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.

2

THE SELECTIVITY OF THE HUMAN PLACENTA IN THE TRANSFER OF PLASMA PROTEINS FROM MOTHER TO FETUS.人胎盘在血浆蛋白从母体向胎儿转移过程中的选择性

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The Fc gamma receptor on human placental plasma membrane. I. Studies on the binding of homologous and heterologous immunoglobulin G1.人胎盘质膜上的Fcγ受体。I. 同源和异源免疫球蛋白G1结合的研究

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Preparation of human placental villous surface membrane.人胎盘绒毛表面膜的制备

Nature. 1974 Nov 22;252(5481):302-3. doi: 10.1038/252302b0.

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Structure and function of immunoglobulin domains. V. Binding, University of immunoglobulin G and fragments to placental membrane preparations.免疫球蛋白结构域的结构与功能。V. 结合，免疫球蛋白G及其片段与胎盘膜制剂的结合

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The Scatchard plot in immunometric assay.免疫分析中的Scatchard图。

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Immunological studies of human placentae: subclass and fragment specificity of binding of aggregated IgG by placental endothelial cells.人胎盘的免疫学研究：胎盘内皮细胞对聚集IgG结合的亚类及片段特异性

Immunology. 1976 Oct;31(4):659-64.

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