Wilce P A
J Membr Biol. 1981;62(3):163-7. doi: 10.1007/BF01998161.
Previous studies have characterized the induction of poly(A)(+)RNA synthesis by aldosterone during the latent period, preceding the increased active transepithelial sodium transport (measured as short-circuit current, SCC). To assess the role of aldosterone in the maintenance of the response in general and the metabolism of this RNA in particular, the decay of the increased SCC and of the newly synthesized poly(A)(+)RNA was monitored. On removal of the hormone, the SCC decayed with a half-life of 6.5 hr after a lag period of 2-3 hr. Studies on the disappearance from the cytoplasm of poly(A)(+)RNA synthesized in the first two hours after addition of aldosterone revealed a number of RNA species with diverse size decaying at a relatively slow rate after removal of aldosterone, and RNA sedimenting in the 10-14 S region decaying at a faster rate closely related to the decay in SCC. Maintenance of aldosterone in the media resulted in a much slower rate of decay of this 10-14 S. It is concluded that the decay of the 10-14 S poly(A)(+)RNA is closely related to the decay in SCC and the stability of this RNA is influenced by the retention of aldosterone in the medium.
以往的研究已经描述了在潜伏期醛固酮诱导多聚腺苷酸（poly(A)）(+)RNA合成的情况，该潜伏期先于主动跨上皮钠转运增加（以短路电流，即SCC来衡量）。为了总体评估醛固酮在维持这种反应中的作用，特别是在这种RNA代谢中的作用，监测了增加的SCC和新合成的多聚腺苷酸（poly(A)）(+)RNA的衰减情况。去除激素后，SCC在2 - 3小时的延迟期后以6.5小时的半衰期衰减。对添加醛固酮后最初两小时内合成的多聚腺苷酸（poly(A)）(+)RNA从细胞质中消失的研究表明，去除醛固酮后，许多大小各异的RNA种类以相对较慢的速率衰减，而在10 - 14 S区域沉降的RNA衰减速率更快，且与SCC的衰减密切相关。培养基中保留醛固酮会导致这种10 - 14 S的衰减速率慢得多。结论是，10 - 14 S多聚腺苷酸（poly(A)）(+)RNA的衰减与SCC的衰减密切相关，并且这种RNA的稳定性受培养基中醛固酮保留情况的影响。
