Wilce P A, Rossier B C, Edelman I S
Biochemistry. 1976 Sep 21;15(19):4279-85. doi: 10.1021/bi00664a022.
Polyadenylated ribonucleic acid [poly(A)(+)-RNA] has been isolated from the cytoplasm of the epithelium of the urinary bladder of the toad (Bufo marinus) by oligo-(deoxythymidylate)cellulose chromatography. Aldosterone increased the incorporation of [3H]uridine, and of [3H]uridine and [3H]adenosine (given in combination) into 7S, 12S, and 18S poly(A)(+)-RNA during the first 30 min of the action of the hormone, as defined by either a pulse or pulse-chase sequence. The quantity of cytoplasmic poly(A)(+)-RNA that hybridized to [3H]poly(uridine) was also increased by aldosterone. These results are consistent with the inference of induction of messenger RNA synthesis. This effect was most marked during the first 30 min of the action of the hormone.
通过寡聚（脱氧胸苷酸）纤维素色谱法，已从蟾蜍（海蟾蜍）膀胱上皮细胞质中分离出聚腺苷酸化核糖核酸[poly(A)(+) -RNA]。醛固酮在激素作用的最初30分钟内，通过脉冲或脉冲追踪序列定义，增加了[3H]尿苷以及[3H]尿苷和[3H]腺苷（联合给予）掺入7S、12S和18S poly(A)(+) -RNA的量。与[3H]聚（尿苷）杂交的细胞质poly(A)(+) -RNA的量也因醛固酮而增加。这些结果与信使RNA合成诱导的推断一致。这种效应在激素作用的最初30分钟最为明显。
