Karawajew L, Fenske H, Böttger M, Lindigkeit R
Mol Biol Rep. 1981 Nov 30;8(1):3-6. doi: 10.1007/BF00798376.
The mass per unit length of 7 X 10(3) (formula: see text) and the corresponding DNA packing ratio of about 14 for the chromatin soluble at moderate ionic strengths has been determined by light scattering. With the increase in ionic strength and corresponding release of histone H1 the DNA packing ratio has been found to decrease down to 4.4. The data obtained are consistent with the idea suggested previously that the salt-soluble chromatin is organized in double nucleosome chains arranged side-by-side and stabilized by H1. With salt-induced H1 release the double chain dissociates and the nucleosomal DNA partially unravels.
通过光散射测定了在中等离子强度下可溶的染色质的每单位长度质量为7×10³（公式：见正文）以及相应的约为14的DNA压缩比。随着离子强度的增加以及组蛋白H1的相应释放，发现DNA压缩比降至4.4。所获得的数据与先前提出的观点一致，即盐溶性染色质由并排排列并由H1稳定的双核小体链组成。随着盐诱导的H1释放，双链解离，核小体DNA部分解开。
