Cheng P T, Pritzker K P, Tausch J, Pittaway A, Millard J
Scan Electron Microsc. 1981(Pt 3):163-8.
We describe the technique and illustrate results of renal calculi analyses utilizing correlative analytical scanning electron microscopy and x-ray microdiffractometry on the same 100 micrometer Spurr embedded sections. Essential to the technique is the employment of an extremely sensitive x-ray microdiffractometer, and for morphological analysis, pretreatment of the sections with a sodium ethoxide etching solution. This technique facilitates more precise identification of crystalline constituents especially calcium apatite and magnesium ammonium phosphate hexahydrate (struvite). Further, the technique permits detection and mapping of both crystalline and amorphous constituents within calculi.
我们描述了一种技术，并展示了利用相关分析扫描电子显微镜和X射线微衍射技术对100微米厚的Spurr包埋切片进行肾结石分析的结果。该技术的关键在于使用极其灵敏的X射线微衍射仪，以及在形态分析方面，用乙醇钠蚀刻溶液对切片进行预处理。这种技术有助于更精确地识别晶体成分，尤其是磷酸钙和六水合磷酸镁铵（鸟粪石）。此外，该技术还能检测结石内的晶体和无定形成分，并进行绘图。
