Richardson C A, Runham N W, Crisp D J
Tissue Cell. 1981;13(4):715-30. doi: 10.1016/s0040-8166(81)80008-0.
The cells of the mantle edge of Cerastoderma edule are described after light and electron microscopical observations. Histochemical tests for calcium in the mantle edge and digestive gland (Dahl, 1952; McGee-Russell, 1958) and analytical electron microscopy of mantle edge of C. edule both failed to show calcium. Similar results were obtained for Mytilus edulis and Chlamys opercularis. However, calcium was detected in the digestive gland of the terrestrial gastropod Helix aspersa. The outer secretory fold of the mantle edge is composed of tall columnar cells. These cells have highly convoluted lateral cell membranes with which many mitochondria are closely associated. These features are indicative of an ion pump which could move calcium from the mantle space to the extrapallial cavity (compare with Bubel's findings, 1973b). There are many features of the cells lining the periostracal groove of C. edule that have not been reported previously (e.g. Bubel, 1973b) and which are now discussed. The periostracal sheet arises within a line of basal cells in the fundus of the periostracal groove. Within these cells the periostracum in section has a spiral form. It is suggested that the newly formed periostracum adheres to the microvillous border through secretions produced from the middle fold cells lining the groove. During its passage along the groove the periostracum is gradually thickened by secretions from the outer fold cells.
在进行光镜和电镜观察后，对欧洲鸟蛤（Cerastoderma edule）外套膜边缘的细胞进行了描述。对外套膜边缘和消化腺进行了钙的组织化学检测（达尔，1952年；麦吉 - 拉塞尔，1958年），并且对欧洲鸟蛤外套膜边缘进行了分析电子显微镜观察，均未发现钙。对紫贻贝（Mytilus edulis）和盖鳃牡蛎（Chlamys opercularis）也得到了类似结果。然而，在陆生腹足动物庭院蜗牛（Helix aspersa）的消化腺中检测到了钙。外套膜边缘的外分泌褶皱由高柱状细胞组成。这些细胞具有高度卷曲的侧细胞膜，许多线粒体与之紧密相连。这些特征表明存在一个离子泵，它可以将钙从外套膜腔转移到外套膜外腔（与布贝尔1973年b的发现相比较）。欧洲鸟蛤围壳沟内衬细胞有许多特征此前未被报道过（例如布贝尔，1973年b），现将予以讨论。围壳片在围壳沟底部的一排基底细胞内产生。在这些细胞内，切片中的围壳呈螺旋状。有人认为，新形成的围壳通过围壳沟内衬中间褶皱细胞产生的分泌物附着在微绒毛边界上。在围壳沿着围壳沟移动的过程中，它会因外套膜外褶皱细胞的分泌物而逐渐增厚。
