培养的哺乳动物细胞的谷胱甘肽及谷胱甘肽S-转移酶活性

Glutathione and glutathione S-transferase activities of mammalian cells in culture.

作者信息

Summer K H, Wiebel F J

出版信息

Toxicol Lett. 1981 Dec;9(4):409-13. doi: 10.1016/0378-4274(81)90018-7.

Abstract

Continuous cell cultures derived from various tissues of rat, mouse, hamster and man were assayed for their glutathione (GSH) content and glutathione S-transferase activities. GSH S-transferase activities were monitored toward the substrates 1-chloro-2,4-dinitro-benzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB) and 1,2-epoxy-3-(p-nitrophenoxy)propane (PO). All cell lines tested contained appreciable amounts of GSH ranging from 10 to 65 nmol/mg cellular protein. Likewise, all cell lines expressed GSH S-transferase activities. However, the various cell lines differed considerably in their relative transferase activities exhibiting some degree of species-specificity.

摘要

对源自大鼠、小鼠、仓鼠和人类各种组织的连续细胞培养物进行了谷胱甘肽（GSH）含量和谷胱甘肽S-转移酶活性的测定。监测了谷胱甘肽S-转移酶对底物1-氯-2,4-二硝基苯（CDNB）、1,2-二氯-4-硝基苯（DCNB）和1,2-环氧-3-（对硝基苯氧基）丙烷（PO）的活性。所有测试的细胞系都含有相当数量的谷胱甘肽，范围为10至65 nmol/mg细胞蛋白。同样，所有细胞系都表达谷胱甘肽S-转移酶活性。然而，各种细胞系的相对转移酶活性差异很大，表现出一定程度的物种特异性。