Carakostas M C, Moore W E, Smith J E
Am J Vet Res. 1981 Apr;42(4):620-2.
A procedure with chromium-51 (51Cr) as the cell label that maintains high-cell viability for studying granulocyte kinetics in horses is described. The procedure combines and modifies several methods for isolating leukocytes and granulocytes for use in the horse when a large volume of labeled cells is required. Also described is an improved technique for measuring granulocyte specific activity in large serial blood samples, using a Ficoll-sedimentation method. The procedure should be useful for determining granulocyte kinetics in the horse, the only major domestic species for which such data are not available.
本文描述了一种以铬-51（51Cr）作为细胞标记的方法，该方法能保持高细胞活力，用于研究马的粒细胞动力学。当需要大量标记细胞时，该方法结合并改进了几种用于分离马白细胞和粒细胞的方法。还描述了一种使用Ficoll沉降法测量大量连续血样中粒细胞比活性的改进技术。该方法对于确定马的粒细胞动力学应该是有用的，马是唯一缺乏此类数据的主要家养物种。
