Racine F M, Vary J C
Prep Biochem. 1982;12(3):265-73. doi: 10.1080/00327488208065567.
An improved method for spore membranes isolation was developed based on sucrose density gradient centrifugation in a vertical rotor. The advantage of this over previous methods was the complete removal of RNA and a 40% reduction in protein content, while retaining the high specific activities for membrane bound dehydrogenases and for amino acid uptake.
基于垂直转子中的蔗糖密度梯度离心法，开发了一种改进的孢子膜分离方法。与以前的方法相比，这种方法的优点是能完全去除RNA，蛋白质含量降低40%，同时保留膜结合脱氢酶和氨基酸摄取的高比活性。
