Soave C, Tardani L, Di Fonzo N, Salamini F
Cell. 1981 Dec;27(2 Pt 1):403-10. doi: 10.1016/0092-8674(81)90423-2.
Zeins, the major endosperm proteins of maize, represent about 50% of total seed proteins and consist of several alcohol-soluble polypeptides encoded by at least four families of genes. The accumulation of zeins is under the control of several broadly defined regulatory genes. One of these, the opaque-2 mutant, lowers the level of zeins, thus improving the nutritional quality of maize meals. We demonstrate here that a salt-soluble protein of molecular weight 32,000 (b-32) is under control of O2. Seven o2 recessive alleles are CRM- when assayed with b-32 antibody. The O2 gene does not encode protein b-32, which apparently is the gene product of the O6 locus. 06 endosperms are CRM- for b-32 protein and are almost devoid of zeins. This indicates that b-32 plays an important role in accumulation of zeins.
醇溶蛋白是玉米胚乳中的主要蛋白质，约占种子总蛋白的50%，由至少四个基因家族编码的几种醇溶性多肽组成。醇溶蛋白的积累受几个广义调控基因的控制。其中之一，即不透明-2突变体，可降低醇溶蛋白水平，从而改善玉米粉的营养品质。我们在此证明，分子量为32,000的盐溶性蛋白（b-32）受O2控制。用b-32抗体检测时，七个o2隐性等位基因呈CRM-。O2基因不编码蛋白b-32，b-32显然是O6位点的基因产物。O6胚乳中b-32蛋白呈CRM-，且几乎不含醇溶蛋白。这表明b-32在醇溶蛋白的积累中起重要作用。
