Cals M J, Adolphe M, Jardillier J C, Miocque M, Agneray J, Ekindjian O G
Int J Tissue React. 1981 Dec;3(3-4):113-9.
Fibroblasts in normal metabolic conditions constitute a simple cellular model for the study of anti-inflammatory drugs in culture, but do not take into account the factors related to the acute inflammatory reaction. A new approach was tried in submitting the fibroblasts to the action of a mouse peritoneal exudate or selected activated macrophages. Disrupted peritoneal material was inoculated to cultures in a volume representing a number of cells corresponding to 25 or 50% of fibroblast concentration at day 0 of the experimental time. Three anti-inflammatory drugs were retained to study their effects on proliferation and metabolic parameters: hydrocortisone, indomethacin and a newly-described molecule, the methoxybenzalthiosemicarbazone (MBT). At day 0, 1, and 2, fibroblasts were counted and total cellular protein and cellular glycolytic activity were determined. 4.5 X 10(-5) M hydrocortisone and MBT protected the fibroblast from the antiproliferative action of peritoneal exudate whereas in experiments using activated macrophage homogenate, all drugs exerted a protective and stimulatory effect.
在正常代谢条件下，成纤维细胞构成了用于研究培养中的抗炎药物的简单细胞模型，但未考虑与急性炎症反应相关的因素。尝试了一种新方法，即将成纤维细胞置于小鼠腹腔渗出液或选定的活化巨噬细胞的作用之下。将破碎的腹腔物质以一定体积接种到培养物中，该体积所代表的细胞数量相当于实验时间第0天成纤维细胞浓度的25%或50%。保留了三种抗炎药物来研究它们对增殖和代谢参数的影响：氢化可的松、吲哚美辛和一种新描述的分子，甲氧基苯硫代半卡巴腙（MBT）。在第0天、第1天和第2天，对成纤维细胞进行计数，并测定总细胞蛋白和细胞糖酵解活性。4.5×10⁻⁵ M的氢化可的松和MBT保护成纤维细胞免受腹腔渗出液的抗增殖作用，而在使用活化巨噬细胞匀浆的实验中，所有药物都发挥了保护和刺激作用。
