Chen B, Chou T H, Sensenbrenner L
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI 48201.
Exp Hematol. 1993 Nov;21(12):1591-6.
Peritoneal injection of thioglycollate medium (TM) to mice results in a dramatic increase in total number of peritoneal macrophages within 48 to 72 hours. Unlike resident macrophages, a fraction (10 to 20%) of these newly arrived young macrophages, designated as macrophage colony-forming cells (M-CFC), are highly proliferative and formed macrophage colonies in vitro in the presence of either macrophage or granulocyte-macrophage colony-stimulating factor (M-CSF or GM-CSF). Using a reverse transcriptase polymerase chain reaction (RT-PCR) technique, peritoneal exudate macrophages (PEM) obtained 2 to 5 days after a single TM injection actively expressed mRNA for recombinant murine macrophage inflammatory protein-1 alpha (rmMIP-1 alpha). Yet none or only a trace amount of mRNA for MIP-1 alpha was detected in normal resident macrophages or PEM obtained 7 days after TM treatment. The effect of rmMIP-1 alpha on the induction of exudate M-CFC was investigated. Multiple intraperitoneal (IP) administration of rmMIP-1 alpha caused a marked increase in the total number of peritoneal M-CFC and macrophages similar to but weaker than the increase in TM-injected mice. The total number of neutrophils, mast cells, and eosinophils also increased, but with different kinetics, following multiple injections of rmMIP-1 alpha. rmMIP-1 alpha alone did not stimulate the proliferation of M-CFC, nor did it potentiate their responsiveness to either rmGM-CSF or recombinant human (rh) M-CSF in vitro. Taken together, our results suggest that MIP-1 alpha released by exudate macrophages is a major chemoattractant responsible for the migration of M-CFC from the circulation to the peritoneal cavity during a TM-induced inflammatory response.
向小鼠腹腔注射巯基乙酸盐培养基（TM）会导致在48至72小时内腹腔巨噬细胞总数急剧增加。与常驻巨噬细胞不同，这些新到达的年轻巨噬细胞中有一部分（10%至20%），被指定为巨噬细胞集落形成细胞（M-CFC），具有高度增殖性，并在巨噬细胞或粒细胞-巨噬细胞集落刺激因子（M-CSF或GM-CSF）存在的情况下在体外形成巨噬细胞集落。使用逆转录酶聚合酶链反应（RT-PCR）技术，在单次注射TM后2至5天获得的腹腔渗出巨噬细胞（PEM）可积极表达重组鼠巨噬细胞炎性蛋白-1α（rmMIP-1α）的mRNA。然而，在正常常驻巨噬细胞或TM处理7天后获得的PEM中未检测到或仅检测到微量的MIP-1α mRNA。研究了rmMIP-1α对渗出液M-CFC诱导的影响。多次腹腔内（IP）给予rmMIP-1α导致腹腔M-CFC和巨噬细胞总数显著增加，与注射TM的小鼠相似但较弱。多次注射rmMIP-1α后，中性粒细胞、肥大细胞和嗜酸性粒细胞的总数也增加，但动力学不同。单独的rmMIP-1α不会刺激M-CFC的增殖，在体外也不会增强它们对rmGM-CSF或重组人（rh）M-CSF的反应性。综上所述，我们的结果表明，渗出巨噬细胞释放的MIP-1α是一种主要的趋化因子，负责在TM诱导的炎症反应期间M-CFC从循环系统迁移到腹腔。
