代谢抑制剂对粘性放线菌胞外果糖基转移酶产生的影响。
Effects of metabolic inhibitors on extracellular fructosyltransferase production in Actinomyces viscosus.
作者信息
Chak W, Kuramitsu H K
出版信息
Infect Immun. 1981 Dec;34(3):930-7. doi: 10.1128/iai.34.3.930-937.1981.
Extracellular fructosyltransferase (levansucrase; EC 2.4.1.10) production in Actinomyces viscosus T14AV was demonstrated to occur concomitantly with cellular growth. The inhibition of both cellular ribonucleic acid and protein synthesis resulted in no further accumulation of enzyme activity. The antibiotic sodium clofibrate differentially inhibited the production of fructosyltransferase by strain T14AV. Furthermore, the antibiotic preferentially inhibited [14C]acetate incorporation into cellular lipid, but did not affect protein synthesis. In addition, no inhibition of fructosyltransferase production was observed upon the addition of the fatty acid acid synthesis inhibitor cerulenin. On the other hand, extracellular fructosyltransferase production was apparently stimulated in the presence of the cell wall synthesis inhibitors penicillin, amphomycin, and tunicamycin. These results are discussed in terms of the mechanism of extracellular protein production in A. viscosus.
已证明粘性放线菌T14AV中细胞外果糖基转移酶（果聚糖蔗糖酶；EC 2.4.1.10）的产生与细胞生长同时发生。细胞核糖核酸和蛋白质合成的抑制均未导致酶活性的进一步积累。抗生素氯贝丁酯钠对T14AV菌株产生果糖基转移酶有不同程度的抑制作用。此外，该抗生素优先抑制[14C]乙酸掺入细胞脂质，但不影响蛋白质合成。另外，添加脂肪酸合成抑制剂浅蓝菌素后未观察到果糖基转移酶产生受到抑制。另一方面，在细胞壁合成抑制剂青霉素、两性霉素和衣霉素存在的情况下，细胞外果糖基转移酶的产生明显受到刺激。根据粘性放线菌中细胞外蛋白质产生的机制对这些结果进行了讨论。
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