Gavin J B, Hjelms E, Barratt-Boyes B G
Pathology. 1981 Oct;13(4):749-56. doi: 10.3109/00313028109086648.
Autografts of pericardium were inserted as patches into incisions across the annulus of the pulmonary valve in 12 adult dogs and 12 pups. In order to accommodate for possible effects of pulmonary hypertension which pertains in many clinical circumstances, constricting teflon bands were placed around the main pulmonary artery in half of the animals. Samples of tissue from the patches were examined by light and scanning electron microscopy from 6 to 14 mth later. The eventual thickness of the insertion site of the patches was found to become 2-14 times greater than the initial autograft due to apposition of layers of collagenous tissue on the endocardial and epicardial surfaces of the autografts. Three pups showed small, angiographically undetected central aneurysms in their patches but the tissue in their base was 5 or more times thicker than control pericardium. Cartilaginous metaplasia was evident at the margins of 57% of the patches, necrotic remnants of parts of the autograft were evident in 38% and foci of dystrophic calcification in 24% of them. Whereas smooth muscle cells sometimes extended into the repair tissue (19%), elastic fibres were not present in the patches. Thus pericardial autografts in the right ventricular outflow tract seem unlikely to develop clinically significant abnormalities.
将心包自体移植物作为补片植入12只成年犬和12只幼犬的肺动脉瓣环切口处。为了适应许多临床情况下存在的肺动脉高压可能产生的影响，在一半的动物的主肺动脉周围放置了收缩性聚四氟乙烯带。6至14个月后，通过光学显微镜和扫描电子显微镜检查补片的组织样本。由于自体移植物的心内膜和心外膜表面有胶原组织层附着，发现补片植入部位的最终厚度比初始自体移植物厚2至14倍。3只幼犬的补片中出现了小的、血管造影未检测到的中央动脉瘤，但动脉瘤底部的组织比对照心包厚5倍或更多倍。57%的补片边缘有软骨化生，38%的补片中可见自体移植物部分的坏死残余物，24%的补片中可见营养不良性钙化灶。虽然平滑肌细胞有时会延伸到修复组织中（19%），但补片中不存在弹性纤维。因此，右心室流出道的心包自体移植物似乎不太可能出现具有临床意义的异常。
