胎儿和母体红细胞免疫黏附（RCIA）受体

Fetal and maternal red cell immune adherence (RCIA) receptors.

作者信息

Siegel I, Gleicher N

出版信息

Am J Reprod Immunol (1980). 1981;1(2):88-91. doi: 10.1111/j.1600-0897.1981.tb00023.x.

DOI:10.1111/j.1600-0897.1981.tb00023.x

Abstract

The addition of autologous serum to mixtures containing human red cells, from pregnant and nonpregnant females, and sheep cells resulted in the formation of mixed aggregates containing both human and sheep red cells. In contrast, no aggregate formation occurred when autologous cord serum was added to mixtures containing cord red cells and sheep red cells. Heat inactivation of the adult serum or the presence of 0.15 M EDTA prevented the formation of mixed aggregates. These observations indicated that the mixed aggregates occurred through the complement-dependent red cell immune adherence (RCIA) phenomenon. The addition of untreated cord serum to mixtures containing inactivated adult serum restored the formation of mixed aggregates, indicating that the cord serum contained sufficient complement for RCIA. Natural antibody against sheep red cells was present in adult sera but was absent in cord sera. Using the RCIA receptor assay, the RCIA receptor activity of cord red cells was found to exceed significantly that of the adult pregnant cells (p less than 0.0025). It is postulated that this may represent an aspect of immune adaptation between mother and fetus.

摘要

将自体血清添加到含有来自孕妇和非孕妇的人类红细胞以及绵羊细胞的混合物中，会导致形成同时包含人类和绵羊红细胞的混合聚集体。相比之下，当将自体脐血清添加到含有脐红细胞和绵羊红细胞的混合物中时，未发生聚集体形成。成人血清的热灭活或0.15 M EDTA的存在可阻止混合聚集体的形成。这些观察结果表明，混合聚集体是通过补体依赖性红细胞免疫黏附（RCIA）现象形成的。将未处理的脐血清添加到含有热灭活成人血清的混合物中可恢复混合聚集体的形成，表明脐血清含有足够的补体用于RCIA。成人血清中存在针对绵羊红细胞的天然抗体，但脐血清中不存在。使用RCIA受体测定法，发现脐红细胞的RCIA受体活性显著超过成年孕妇红细胞的活性（p小于0.0025）。据推测，这可能代表了母婴之间免疫适应的一个方面。