Licence S T, Binns R M
Vet Immunol Immunopathol. 1984 Oct;7(3-4):255-73. doi: 10.1016/0165-2427(84)90084-9.
Sheep antibodies against a pig E-rosette-forming lymphoblastic T lymphoma raised by two intravenous injections of 10(10) cells showed little lymphocytotoxic activity which could be absorbed with red cells, alveolar macrophages or kidney or liver cell homogenates. Bone marrow absorption yielded subpopulation specific antibody which binds to E rosette-forming cells (E.RFC) using either complement-mediated cytotoxicity or indirect antiglobulin rosette formation. In 30 blood lymphocyte preparations from 20 pigs with a range of approximately 20-85% E rosettes the mean E% 43.5 +/- 2.7 agreed with the % antigen+ cells by cytotoxicity mean = 42.6 +/- 2.7 and in each individual sample these figures also agreed closely. In samples of blood lymphocytes enriched and depleted for E rosettes, results of %E+ also agreed closely with % antigen+ cells. This relationship also held for thymocytes and the specific antibodies could be completely absorbed with thymocytes. These data show that the antibody identified peripheral and thymic E.RFC. Bound to lymphocytes the antibody inhibited E rosette-formation with sheep red blood cells (SRBC) in saline (S) and dextran (DS) and with pig RBC in dextran and in Ficoll, but did not affect B cells shown by immunofluorescence, direct antiglobulin rosette formation or Fc rosette-formation, either in saline or dextran, (which include T gamma cells). E rosette inhibition was dependent on antibody concentration, showing single and double sigmoid curves for S and DS rosettes respectively, consistent with differing ease of inhibition of the strong and weak rosette formation. The same spectrum of inhibition of rosette formation by antibody binding followed subsequent incubation with C'6-deficient rabbit serum, but with C'-sufficient serum resulted in loss of cells which require dextran for Fc rosette-formation (T gamma). Thus the serum reveals E rosette-forming T cells and their subpopulations, perhaps by binding to the SRBC receptor.
通过两次静脉注射10¹⁰个细胞产生的针对猪E花环形成淋巴细胞性T淋巴瘤的绵羊抗体，几乎没有可被红细胞、肺泡巨噬细胞或肾或肝细胞匀浆吸收的淋巴细胞毒性活性。骨髓吸收产生了亚群特异性抗体，该抗体使用补体介导的细胞毒性或间接抗球蛋白花环形成与E花环形成细胞（E.RFC）结合。在来自20头猪的30份血淋巴细胞制剂中，E花环范围约为20 - 85%，平均E%为43.5±2.7，与细胞毒性法测得的抗原阳性细胞百分比平均值42.6±2.7相符，且在每个个体样本中这些数字也非常接近。在富集和去除E花环的血淋巴细胞样本中，E⁺%的结果也与抗原阳性细胞百分比非常接近。这种关系在胸腺细胞中也成立，并且特异性抗体可被胸腺细胞完全吸收。这些数据表明该抗体识别外周和胸腺E.RFC。与淋巴细胞结合后，该抗体抑制了在盐水（S）和右旋糖酐（DS）中与绵羊红细胞（SRBC）以及在右旋糖酐和聚蔗糖中与猪红细胞形成E花环，但不影响通过免疫荧光、直接抗球蛋白花环形成或Fc花环形成所显示的B细胞，无论是在盐水中还是在右旋糖酐中（包括Tγ细胞）。E花环抑制取决于抗体浓度，分别显示出S和DS花环的单 sigmoid 曲线和双 sigmoid 曲线，这与强弱花环形成抑制的难易程度不同一致。抗体结合对花环形成的抑制谱在随后与C'6缺陷兔血清孵育后相同，但与C'充足的血清孵育导致需要右旋糖酐进行Fc花环形成的细胞（Tγ）丢失。因此，该血清可能通过与SRBC受体结合揭示了E花环形成T细胞及其亚群。
