Ototani N, Yosizawa Z
J Biochem. 1981 Nov;90(5):1553-6. doi: 10.1093/oxfordjournals.jbchem.a133625.
Porcine intestinal heparin was partially digested with a purified heparinase and an octasaccharide with high-affinity for antithrombin III was isolated from the digest by gel filtration, followed by affinity chromatography on a column of Sepharose 4B coupled with antithrombin III. The anticoagulant activity determined by the activated partial thromboplastin time method of the octasaccharide was 240 units/mg. Fifty percent inactivation activities of the octasaccharide for thrombin and factor Xa in the presence of antithrombin III were 2 and 6.5 times, respectively, higher than those of the initial heparin. These data indicate that the octasaccharide possesses the critical structural integrities required for manifesting these biological activities.
用纯化的肝素酶对猪肠肝素进行部分消化，通过凝胶过滤从消化产物中分离出对抗凝血酶III具有高亲和力的八糖，随后在偶联有抗凝血酶III的琼脂糖4B柱上进行亲和层析。通过活化部分凝血活酶时间法测定的该八糖的抗凝血活性为240单位/毫克。在抗凝血酶III存在下，该八糖对凝血酶和因子Xa的50%失活活性分别比初始肝素高2倍和6.5倍。这些数据表明，该八糖具有表现这些生物活性所需的关键结构完整性。
