Department of Chemistry, University of California, Davis, California 95616, USA.
Anal Chem. 2012 Mar 6;84(5):2416-23. doi: 10.1021/ac203190k. Epub 2012 Feb 14.
Heparin is a linear sulfated polysaccharide widely used in medicine because of its anticoagulant properties. The various sulfation and/or acetylation patterns on heparin impart different degrees of conformational change around the glycosidic bonds and subsequently alter its function as an anticoagulant, anticancer, or antiviral drug. Characterization of these structures is important for eventual elucidation of its function but presents itself as an analytical challenge due to the inherent heterogeneity of the carbohydrates. Heparin octasaccharide structural isomers of various sulfation patterns were investigated using ion mobility mass spectrometry (IMMS). In addition to distinguishing the isomers, we report the preparation and tandem mass spectrometry analysis for multiple sulfated or acetylated oligosaccharides. Herein, our data indicate that heparin octasaccharide isomers were separated on the basis of their structural conformations in the ion mobility cell. Subsequent to this separation, isomers were further distinguished using product ions resulting from tandem mass spectrometry. Overall, IMMS analysis was used to successfully characterize and separate individual isomers and subsequently measure their conformations.
肝素是一种线性硫酸化多糖，由于其抗凝特性，在医学中被广泛应用。肝素上的各种硫酸化和/或乙酰化模式赋予糖苷键周围不同程度的构象变化，从而改变其作为抗凝剂、抗癌剂或抗病毒药物的功能。这些结构的表征对于最终阐明其功能很重要，但由于碳水化合物的固有异质性，这给分析带来了挑战。本研究采用离子淌度质谱（IMMS）研究了具有不同硫酸化模式的肝素八糖结构异构体。除了区分异构体之外，我们还报告了多种硫酸化或乙酰化寡糖的制备和串联质谱分析。本文数据表明，肝素八糖异构体是根据其在离子淌度池中结构构象进行分离的。在这种分离之后，使用串联质谱产生的产物离子进一步区分异构体。总之，IMMS 分析成功地用于对单个异构体进行特征分析和分离，并随后测量其构象。
