An improved method to study antibacterial activity of antibiotics in an in vitro model simulating serum levels.

The bacterial activity of cefoxitin was studied in an improved in vitro model simulating serum levels. The peak concentration of cefoxitin was 110 mcg/ml as achieved after 1 g i.v. Half-life time was adjusted to 50 minutes. The time at which the viable count reached the mark 1% of the original inoculum after the lowest count was determined (regrowth period) ranged from one to 10 hours from the beginning of the experiments.