景天酸代谢植物中的过氧化物酶体和线粒体柠檬酸合酶。

Peroxisomal and mitochondrial citrate synthase in CAM plants.

作者信息

Zafra M F, Segovia J L, Alejandre M J, García-Peregrín E

出版信息

Rev Esp Fisiol. 1981 Dec;37(4):491-6.

Abstract

Citrate synthase wa studied for the first time in peroxisomes and mitochondria of crassulacean acid metabolism plants. Cellular organelles were isolated from Agave americana leaves by sucrose density gradient centrifugation and characterized by the use of catalase and cytochrome oxidase as marker enzymes, respectively. 48,000 X g centrifugation caused the breakdown of the cellular organelles. The presence of a glyoxylate cycle enzyme (citrate synthase) and a glycollate pathway enzyme (catalase) in the same organelles, besides the absence of another glyoxalate cycle enzyme (malate synthase) is reported for the first time, suggesting that peroxisomal and glyoxysomal proteins are synthesized at the same time and housed in he same organelle.

摘要

首次在景天酸代谢植物的过氧化物酶体和线粒体中对柠檬酸合酶进行了研究。通过蔗糖密度梯度离心从龙舌兰叶片中分离出细胞器，并分别使用过氧化氢酶和细胞色素氧化酶作为标记酶对其进行表征。48,000×g离心导致细胞器破裂。首次报道在同一细胞器中存在乙醛酸循环酶（柠檬酸合酶）和乙醇酸途径酶（过氧化氢酶），同时不存在另一种乙醛酸循环酶（苹果酸合酶），这表明过氧化物酶体和乙醛酸循环体蛋白是同时合成并存在于同一细胞器中的。