Parish R W
Arch Microbiol. 1975 Nov 7;105(3):187-92. doi: 10.1007/BF00447136.
Peroxisomes were isolated form derepressed (lactose grown) Saccharomyces cerevisiae cells following homogenization with a "Merkenschlager" cell mill (at 0 degrees C using glass beads). Catalase and urate oxidase, along with low activities of D-amino acid oxidase and L-alpha-hydroxyacid oxidase (glycollate oxidase), were associated with the peroxisomes. No catalase activity was present in glucose repressed cells. When protoplasts prepared from derepressed cells were used for peroxisome isolation, catalase activity was not sedimentable through gradients. Apparently peroxisomes were destroyed as the cells became fermentative during protoplast preparation. The distribution of glyoxylate cycle enzymes was examined. Isocitrate lyase was not sedimentable, suggesting that, if the enzyme is peroxisome-associated, it is either readily released of present in a labile second class of peroxisomes. Low activities of malate dehydrogenase and citrate synthetase were found in peroxisome fractions from gradients, but may represent mitochondrial contamination. Citrate synthetase was not found associated with a low-density particle as had been previously reported.
过氧化物酶体是从去阻遏（乳糖培养）的酿酒酵母细胞中分离得到的，使用“Merkenschlager”细胞研磨机（在0℃下使用玻璃珠）进行匀浆。过氧化氢酶和尿酸氧化酶，以及低活性的D-氨基酸氧化酶和L-α-羟基酸氧化酶（乙醇酸氧化酶）与过氧化物酶体相关。在葡萄糖阻遏的细胞中不存在过氧化氢酶活性。当使用从去阻遏细胞制备的原生质体进行过氧化物酶体分离时，过氧化氢酶活性不能通过梯度沉降。显然，在原生质体制备过程中，随着细胞变成发酵型，过氧化物酶体被破坏。对乙醛酸循环酶的分布进行了检查。异柠檬酸裂解酶不能沉降，这表明，如果该酶与过氧化物酶体相关，它要么很容易释放，要么存在于不稳定的第二类过氧化物酶体中。在梯度分离得到的过氧化物酶体组分中发现了低活性的苹果酸脱氢酶和柠檬酸合酶，但可能代表线粒体污染。未发现柠檬酸合酶与先前报道的低密度颗粒相关。
