在缓激肽（BK）存在的情况下，1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）标记血小板的稳态荧光偏振（FP）
Steady-state fluorescence polarization (FP) of 1,6-diphenyl-1,3,5-hexatriene (dph)-labelled platelets in the presence of bradykinin (bk).
作者信息
Stahl K W, Mishal Z
出版信息
Agents Actions. 1981 Dec;11(6-7):659-61. doi: 10.1007/BF01978785.
Steady-state fluorescence polarization (FP) decreases, when 1,6-diphenyl-1,3,5-hextriene (dph) labelled platelets are exposed to bradykinin (bk). At pH 8, the dose-response curve is bell-shaped with an optimum bk effect at 10(-7) M. In contrast to the ricinoleic-acid ester of glycerin-polyethyleneglycol, cremophor EL (CEL), bk is no more effective when platelets are pretreated with 10(-5) M p-bromophenacylbromide (B phi B). These results suggest that platelets are target cells for the peptide bk, which induces an FP decrease indirectly by stimulating the release of non-saturated fatty acids in the platelet membrane.
当用1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）标记的血小板暴露于缓激肽（BK）时，稳态荧光偏振（FP）降低。在pH 8时，剂量 - 反应曲线呈钟形，在10⁻⁷ M时BK效应最佳。与甘油 - 聚乙二醇的蓖麻油酸酯（聚氧乙烯蓖麻油，CEL）不同，当血小板用10⁻⁵ M对溴苯甲酰溴（BϕB）预处理时，BK不再有效。这些结果表明血小板是肽BK的靶细胞，BK通过刺激血小板膜中不饱和脂肪酸的释放间接诱导FP降低。
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