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基底膜糖蛋白层粘连蛋白与糖胺聚糖的结合。一项亲和层析研究。

Binding of the basement-membrane glycoprotein laminin to glycosaminoglycans. An affinity-chromatography study.

作者信息

Del Rosso M, Cappelletti R, Viti M, Vannucchi S, Chiarugi V

出版信息

Biochem J. 1981 Dec 1;199(3):699-704. doi: 10.1042/bj1990699.

Abstract

The binding of the basement-membrane glycoprotein laminin to glycosaminoglycans (aggregating and non-aggregating subsets of heparan sulphates and dermatan sulphates, as well as heparin, chondroitin sulphates and hyaluronic acid) was studied by affinity chromatography. Partially periodate-oxidized chains of glycosaminoglycans were coupled to adipic acid dihydrazide-substituted agarose. Co-polymeric glycosaminoglycans reveal high affinity for laminin, whereas hyaluronic acid does not. Competitive-release experiments indicate that glycosaminoglycans share a common binding site on the laminin molecule.

摘要

通过亲和色谱法研究了基底膜糖蛋白层粘连蛋白与糖胺聚糖(硫酸乙酰肝素和硫酸皮肤素的聚集和非聚集亚组,以及肝素、硫酸软骨素和透明质酸)的结合。将部分高碘酸盐氧化的糖胺聚糖链与己二酸二酰肼取代的琼脂糖偶联。共聚糖胺聚糖对层粘连蛋白显示出高亲和力,而透明质酸则不然。竞争性释放实验表明,糖胺聚糖在层粘连蛋白分子上共享一个共同的结合位点。

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