Miyahara T, Torisu M
Gan. 1981 Dec;72(6):854-61.
The effect of sera from 107 patients with gastrointestinal cancer on macrophage chemotaxis was studied using a modified Boyden chamber technique. A macrophage chemotactic inhibitory factor (CIF) was found in sera of advanced cancer patients. The CIF activity was significantly stage-related, and increased especially in patients with negative cutaneous responsiveness in purified protein derivative, dinitrochlorobenzene and keyhole limpet hemocyanin skin tests, and with low circulating T-lymphocyte levels. In addition, the CIF was found to suppress neutrophil as well as macrophage chemotaxis. suppress neutrophil as well as macrophage chemotaxis. The mode of action and the characteristics of the CIF were investigated, and the following results were obtained. (1) The CIF directly interacted with macrophages and neutrophils and suppressed their chemotactic activities without apparent cytotoxic effect on these cells. (2) On Pevikon block electrophoresis, the CIF activity was most prominent in the alpha-globulin region, although considerable heterogeneity and broadness of the CIF bands were observed. (3) Dialysis and lyophilization had no effects on the CIF activity. On heating at 56 degree for 30 min, the CIF activity seemed to be decreased. This study indicates that CIF in cancer patients may be detrimental to the host immune defense against tumor growth by producing defects of macrophage and neutrophil chemotaxis in vivo.
采用改良的Boyden小室技术，研究了107例胃肠道癌患者血清对巨噬细胞趋化性的影响。在晚期癌症患者的血清中发现了一种巨噬细胞趋化抑制因子（CIF）。CIF活性与疾病分期显著相关，尤其在纯化蛋白衍生物、二硝基氯苯和钥孔戚血蓝蛋白皮肤试验中皮肤反应阴性且循环T淋巴细胞水平较低的患者中升高。此外，发现CIF可抑制中性粒细胞以及巨噬细胞的趋化性。对CIF的作用方式和特性进行了研究，获得了以下结果。（1）CIF直接与巨噬细胞和中性粒细胞相互作用，抑制它们的趋化活性，而对这些细胞无明显细胞毒性作用。（2）在Pevikon区带电泳中，CIF活性在α-球蛋白区最为显著，尽管观察到CIF条带存在相当大的异质性和宽度。（3）透析和冻干对CIF活性无影响。在56℃加热30分钟，CIF活性似乎降低。本研究表明，癌症患者体内的CIF可能通过在体内造成巨噬细胞和中性粒细胞趋化性缺陷，对宿主抵抗肿瘤生长的免疫防御产生不利影响。
