Ueda K, Kambara T
Am J Pathol. 1978 Jul;92(1):241-52.
Macrophage-chemotactic factors were extracted from delayed hypersensitivity skin lesions induced by bovine gamma-globulin in guinea pigs. The most active factor, MCFS--1, was highly purified and found to be a heat-labile protein with a molecular weight of 150,000 and to possess in vivo as well as in vitro activity. This factor was homogeneous during polyacrylamide gel electrophoresis, and the chemotactic activity was associated exclusively with this band. Further characterization revealed that its isoelectric point was 6.7 to 6.9 and made a single arc in the beta-globulin region with rabbit antiserums against guinea pig serum on immunoelectrophoresis. This factor seemed to be antigenically different from immunoglobulin G (IgG) by immunodiffusion and immunoadsorption. On the other hand, the chemotactic activity of MCFS-2 was adsorbed by neither anti-IgG nor anti MCFS-1 and that of euglobulin fraction was partially adsorbed by anti-IgG. These indicate the presence of at least three types of antigenically different chemotactic factors for macrophages in the extracts of delayed hypersensitivity skin lesions.
巨噬细胞趋化因子是从豚鼠中由牛γ球蛋白诱导产生的迟发型超敏皮肤损伤中提取的。最具活性的因子MCFS - 1经过高度纯化，发现是一种热不稳定蛋白，分子量为150,000，具有体内和体外活性。该因子在聚丙烯酰胺凝胶电泳中呈均一性，趋化活性仅与该条带相关。进一步的特性分析表明，其等电点为6.7至6.9，在免疫电泳中与兔抗豚鼠血清在β球蛋白区域形成单一弧线。通过免疫扩散和免疫吸附，该因子似乎在抗原性上与免疫球蛋白G（IgG）不同。另一方面，MCFS - 2的趋化活性既不被抗IgG吸附，也不被抗MCFS - 1吸附，而优球蛋白组分的趋化活性部分被抗IgG吸附。这些表明在迟发型超敏皮肤损伤提取物中存在至少三种抗原性不同的巨噬细胞趋化因子。
