Watanabe H K, Hashimoto Y
Gan. 1981 Dec;72(6):930-6.
The ability of carcinogenic and non-carcinogenic aminoazo dyes to induce unscheduled DNA synthesis (UDS) in primary cultures of hepatocytes of 3 strains of rats and 2 strains of mice was examined by means of microautoradiography. Azo dyes tested were 4-aminoazobenzene (AAB) and 4 of its ring methoxyl (MeO) derivatives, their N-hydroxy (N-OH) derivatives, N-methyl-4-aminoazobenzene (MAB) and its N-bezoyloxy derivative, and N,N-dimethyl-4-aminoazobenzene (DAB). All carcinogenic aminoazo dyes (AAB, 3-MeO-AAB, 4'-MeO-AAB, MAB and DAB), but not the non-carcinogenic dyes (2-MeO-AAB and 2,5-diMeO-AAB), induced UDS in rat hepatocytes as well as mouse hepatocytes. N-Hydroxy derivatives of AAB and its 3- and 4'-MeO derivatives elicited higher levels of UDS than did the corresponding mother aminoazo dyes. N-OH-2-MeO-AAB was as inactive with rat hepatocytes as the mother dye. N-OH-2,5-diMeO-AAB, however, elicited a definite level of UDS, in contrast to the mother 2,5-diMeO-AAB. N-Benzoyloxy-MAB, a prototype compound of the ultimate carcinogenic metabolite of MAB, was as active as the mother MAB. N,-OH-3-MeO-AAB induced UDS more rapidly than did 3-MeO-AAB. The UDS elicited by 3-MeO-AAB was found to end within a few hours after releasing the hepatocytes from the azo dye.
通过微放射自显影法检测了致癌和非致癌氨基偶氮染料在3个大鼠品系和2个小鼠品系的原代肝细胞培养物中诱导非预定DNA合成（UDS）的能力。所检测的偶氮染料有4-氨基偶氮苯（AAB）及其4种环甲氧基（MeO）衍生物、它们的N-羟基（N-OH）衍生物、N-甲基-4-氨基偶氮苯（MAB）及其N-苯甲酰氧基衍生物，以及N，N-二甲基-4-氨基偶氮苯（DAB）。所有致癌性氨基偶氮染料（AAB、3-MeO-AAB、4'-MeO-AAB、MAB和DAB），而非致癌染料（2-MeO-AAB和2,5-二MeO-AAB），均可在大鼠肝细胞和小鼠肝细胞中诱导UDS。AAB及其3-和4'-MeO衍生物的N-羟基衍生物诱导的UDS水平高于相应的母体氨基偶氮染料。N-OH-2-MeO-AAB对大鼠肝细胞的活性与母体染料相同。然而，与母体2,5-二MeO-AAB相比，N-OH-2,5-二MeO-AAB可诱导一定水平的UDS。MAB最终致癌代谢产物的原型化合物N-苯甲酰氧基-MAB与母体MAB活性相同。N,-OH-3-MeO-AAB诱导UDS的速度比3-MeO-AAB更快。发现3-MeO-AAB诱导的UDS在将肝细胞从偶氮染料中释放后数小时内结束。
