Williams G M
Natl Cancer Inst Monogr. 1981 Dec(58):237-42.
Liver-derived intact cell systems are useful for examination of the genetic effects of N-substituted aryl compounds. With freshly isolated hepatocytes, the hepatocyte primary culture-DNA repair test detects the genotoxicity of a spectrum of activation-dependent aryl amines and amides. Likewise, continuous lines of adult rat liver epithelial cells are mutated at the hypoxanthine-guanine phosphoribosyl locus by such compounds. Hepatocyte-mediated mutagenesis enhances the activation of aromatic amines to mutagenic metabolites. These intact cell systems provide a balance of detoxification and activation reactions for evaluation of tissue profiles of metabolism and capability for the production of genotoxic metabolites.
肝脏来源的完整细胞系统可用于检测N-取代芳基化合物的遗传效应。通过新鲜分离的肝细胞，肝细胞原代培养-DNA修复试验可检测一系列依赖激活的芳基胺和酰胺的遗传毒性。同样，成年大鼠肝上皮细胞系会被此类化合物在次黄嘌呤-鸟嘌呤磷酸核糖基位点诱导突变。肝细胞介导的诱变作用增强了芳香胺向诱变代谢物的激活过程。这些完整细胞系统为评估代谢的组织特征和产生遗传毒性代谢物的能力提供了解毒和激活反应的平衡。
