Dénes L, Falus A, Böhm U, Merétey K
Acta Physiol Acad Sci Hung. 1981;58(1):21-9.
Immunological disorders may cause altered motility or attachment of leukocytes. Active adherence of buffy cost cells derived from normal subjects and with autoimmune diseases patients and complement dependency of this process was studied on glass surfaces. The adherence of leukocytes of both normal persons and of SLE and RA patients was decreased in the presence of normal human serum compared to serum-free medium. The effects of native and heat inactivated human sera on leukocytes of SLE and RA patients were compared. Adherence of SLE leukocytes was higher, whereas that of RA leukocytes was lower in unheated serum compared to heat inactivated human serum. Supernatants of leukocytes of SLE, RA patients and control subjects were harvested after incubation in serum-free medium. The C3 levels of supernatants were determined by rocket electrophoresis. Surface attached C3 was detected with immunofluorescence mainly on SLE leukocytes. The cellular effects of supernatants on control leukocytes, were consistent with the changes observed with leukocytes derived from SLE, RA or control patients.
免疫紊乱可能导致白细胞运动性或黏附性改变。研究了正常受试者及自身免疫性疾病患者的血沉棕黄层细胞在玻璃表面的主动黏附以及该过程的补体依赖性。与无血清培养基相比，在正常人血清存在的情况下，正常人和系统性红斑狼疮（SLE）及类风湿关节炎（RA）患者的白细胞黏附性均降低。比较了天然和热灭活的人血清对SLE和RA患者白细胞的影响。与热灭活的人血清相比，未加热血清中SLE患者白细胞的黏附性较高，而RA患者白细胞的黏附性较低。SLE、RA患者及对照受试者的白细胞在无血清培养基中孵育后收集上清液。通过火箭电泳测定上清液中的C3水平。主要在SLE患者白细胞上通过免疫荧光检测到表面附着的C3。上清液对对照白细胞的细胞效应与SLE、RA或对照患者白细胞所观察到的变化一致。
