Heisey G B, Sankaran V
Lab Anim Sci. 1981 Dec;31(6):723-5.
Several techniques were compared for collection of peritoneal mononuclear phagocytes from cynomolgus monkeys. A multiple-holed cannula proved superior to a hypodermic needle for the collection of peritoneal washings because it was not occluded by the omentum. With the cannula technique 55--70% of the harvest medium could be recovered from the peritoneal cavity with a yield of 2.5--5 x 10(5) macrophages per ml. A closed system for injecting and collecting harvest medium was better than a syringe in preventing bacterial contamination of peritoneal washings. After collection, cells were resuspended in growth medium to a concentration of 5 x 10(5) macrophages per ml, distributed into Leighton tubes, and incubated at 36 degrees C. After 12 hours, cultures were washed to remove non-adherent cells and refed with growth medium. This technique provided a sterile, reliable method for the collection and cultivation of macrophages from monkeys.
为了从食蟹猴收集腹腔单核吞噬细胞，对几种技术进行了比较。事实证明，多孔套管在收集腹腔灌洗液方面优于皮下注射针，因为它不会被大网膜堵塞。采用套管技术，55%至70%的收集培养基可从腹腔回收，每毫升产量为2.5至5×10⁵个巨噬细胞。在防止腹腔灌洗液受到细菌污染方面，用于注入和收集收集培养基的封闭系统比注射器更好。收集后，将细胞重悬于生长培养基中，使其浓度达到每毫升5×10⁵个巨噬细胞，分配到莱顿管中，并在36℃下孵育。12小时后，洗涤培养物以去除未贴壁细胞，并用生长培养基重新培养。该技术为从猴子收集和培养巨噬细胞提供了一种无菌、可靠的方法。
