Tschiftschiisky S
Gegenbaurs Morphol Jahrb. 1976;122(4):586-602.
For the obtainment of reactive peritoneal macrophages was used an original technique by which small oval x-ray plates, wrapped up in viteline membrane were grafted into the abdominal cavity of white rats. As a result of the inflammatory reaction reactive cells were adhering to the membrane and among them macrophages of various stages of development as well. The membranes with the reactive cells which have been removed out at different periods of time (6, 12, and 24 h, 3, 5, and 7 d) were embedded in durcupan for electronmicroscopic examination. The ultrastructural organization of the macrophages having different stages of differentiation has been demonstrated by means of this technique. The problem concerning the origin of macrophages coming chiefly of the monocyte migration from blood circulation and passing through various transient forms was discussed as well as their ability to be transformed into giant cells, fibroblasts, mast cells, plasmocytes etc. in the course of the inflammatory reaction.
为获取反应性腹膜巨噬细胞，采用了一种原创技术，即将包裹在卵黄膜中的小椭圆形X线片植入白色大鼠腹腔。炎症反应的结果是反应性细胞附着在膜上，其中也有不同发育阶段的巨噬细胞。在不同时间段（6、12和24小时，3、5和7天）取出带有反应性细胞的膜，将其包埋在环氧树脂中进行电子显微镜检查。通过该技术已证明了不同分化阶段巨噬细胞的超微结构组织。还讨论了巨噬细胞主要起源于血液循环中单核细胞迁移并经历各种过渡形式的问题，以及它们在炎症反应过程中转化为巨细胞、成纤维细胞、肥大细胞、浆细胞等的能力。
