一种从牛外周血白细胞中获取连续细胞系的简单方法。

A simple procedure to obtain continuous cell lines from bovine peripheral blood leucocytes.

作者信息

Asagba M O, Ssentongo Y K, Johnson R H, Smith J R

出版信息

Vet Immunol Immunopathol. 1981 Feb;2(1):87-94. doi: 10.1016/0165-2427(81)90041-6.

Abstract

A method is described by which cell lines can be readily developed from bovine peripheral leucocytes. Fifteen cell lines have been developed from 25 attempts, passage levels up to 60 being reached. The cell lines are aneuploid and predominantly epithelial, show split ratio capabilities of 1:4 to give monolayers with 5 days of routine passage, and have high resistance to laboratory contamination with bacterial and fungal agents. Data are given concerning establishment, morphology, viral susceptibility and chromosomal counts of established cell lines.

摘要

本文描述了一种能从牛外周血白细胞中轻松培养细胞系的方法。在25次尝试中已成功培养出15个细胞系，传代次数达60次。这些细胞系为非整倍体，主要是上皮细胞，分裂比为1:4，常规传代5天可形成单层，且对细菌和真菌试剂造成的实验室污染具有高度抗性。文中给出了有关已建立细胞系的建立、形态、病毒敏感性和染色体计数的数据。