Landsverk T
Acta Vet Scand. 1981;22(3-4):449-58. doi: 10.1186/BF03548670.
Selected enzymes were examined in the small intestine of twelve 2–5 week-old calves, 8 with diarrhea and 4 convalescents. The diarrheic calves showed a reduction of enzyme reactions mainly in the duodenum and middle small intestine, and the crypt reactions appeared most severely affected. In the duodenum, villous alkaline phosphatase, adenosine triphosphate-(ATP)-splitting enzyme, and β-D-galactosidase were reduced in 3 calves; the reaction in the corresponding crypts was decreased in 6 calves for the ATP-splitting enzyme and in 4 calves for the β-D-galactosidase. Six calves showed decrease of villous brush border acid phosphatase, and 3 of villous non-specific esterase. In the middle jejunum, villous ATP-splitting enzyme was reduced in 3 calves, while 5 showed decrease of the corresponding crypt reaction. Convalescents had no enzyme reduction in the duodenum, whereas 1 showed marked reduction of the ATP-splitting enzyme and aminopeptidase in the middle and posterior jejunum. The decreased enzyme reactions in the present material may be caused by immaturity of epithelial cells associated with regenerative crypt hyperplasia and/or microbial destruction of enzymes.
对12头2至5周龄的小牛的小肠中的特定酶进行了检测，其中8头患有腹泻，4头处于恢复期。腹泻小牛的酶反应主要在十二指肠和小肠中部减少，隐窝反应受影响最为严重。在十二指肠中，3头小牛的绒毛碱性磷酸酶、三磷酸腺苷（ATP）分解酶和β-D-半乳糖苷酶减少；6头小牛的ATP分解酶和4头小牛的β-D-半乳糖苷酶在相应隐窝中的反应降低。6头小牛的绒毛刷状缘酸性磷酸酶减少，3头小牛的绒毛非特异性酯酶减少。在空肠中部，3头小牛的绒毛ATP分解酶减少，而5头小牛的相应隐窝反应降低。恢复期小牛的十二指肠中酶没有减少，而1头小牛在空肠中部和后部的ATP分解酶和氨肽酶显著减少。本材料中酶反应的降低可能是由于与再生性隐窝增生相关的上皮细胞不成熟和/或酶的微生物破坏所致。
