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1
Binding of 3H-ADTN, a dopamine agonist, to membranes of the bovine retina.多巴胺激动剂3H-ADTN与牛视网膜膜的结合。
Cell Mol Neurobiol. 1981 Jun;1(2):167-74. doi: 10.1007/BF00710717.
2
Properties of dopamine agonist and antagonist binding sites in mammalian retina.哺乳动物视网膜中多巴胺激动剂和拮抗剂结合位点的特性。
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Striatal binding of 2-amino-6,7-[3H]dihydroxy-1,2,3,4-tetrahydronaphthalene to two dopaminergic sites distinguished by their low and high affinity for neuroleptics.2-氨基-6,7-[3H]二羟基-1,2,3,4-四氢萘在纹状体与两个多巴胺能位点的结合，这两个位点对神经阻滞剂具有低亲和力和高亲和力。
J Neurosci. 1982 Jul;2(7):895-906. doi: 10.1523/JNEUROSCI.02-07-00895.1982.
4
Binding of [3H]-muscimol, a potent gamma-aminobutyric acid receptor agonist, to membranes of the bovine retina.强效γ-氨基丁酸受体激动剂[3H]-蝇蕈醇与牛视网膜膜的结合。
Br J Pharmacol. 1980;71(1):259-64. doi: 10.1111/j.1476-5381.1980.tb10934.x.
5
Pharmacology of high-affinity binding of [3H](+/-)2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene (ADTN) to bovine caudate nucleus tissue.[3H](±)2-氨基-6,7-二羟基-1,2,3,4-四氢萘（ADTN）与牛尾状核组织高亲和力结合的药理学研究
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6
Similar binding of 3H-ADTN and 3H-apomorphine to calf brain dopamine receptors.3H-ADTN和3H-阿扑吗啡与小牛脑多巴胺受体的相似结合。
Eur J Pharmacol. 1979 Apr 15;55(2):137-42. doi: 10.1016/0014-2999(79)90385-6.
7
Characterization of dopamine receptors by 3H-ADTN binding in calf adrenal zona glomerulosa.
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8
Identification of dopamine "D3" and "D4" binding sites, labelled with [3H]2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene, as high agonist affinity states of the D1 and D2 dopamine receptors, respectively.分别鉴定用[³H]2-氨基-6,7-二羟基-1,2,3,4-四氢萘标记的多巴胺“D3”和“D4”结合位点，作为D1和D2多巴胺受体的高激动剂亲和力状态。
J Neurochem. 1986 Apr;46(4):1058-67. doi: 10.1111/j.1471-4159.1986.tb00618.x.
9
[3H] 2-Amino-6,7-dihydroxy 1,2,3,4-tetrahydronapthalene (ADTN): a potential specific dopamine receptor ligand.[3H]2-氨基-6,7-二羟基-1,2,3,4-四氢萘（ADTN）：一种潜在的特异性多巴胺受体配体。
Res Commun Chem Pathol Pharmacol. 1978 Oct;22(1):15-25.
10
Sodium ion modulates D2 receptor characteristics of dopamine agonist and antagonist binding sites in striatum and retina.钠离子调节纹状体和视网膜中多巴胺激动剂和拮抗剂结合位点的D2受体特性。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4212-6. doi: 10.1073/pnas.79.13.4212.

引用本文的文献

1
Retinal dopamine D1 and D2 receptors: characterization by binding or pharmacological studies and physiological functions.视网膜多巴胺D1和D2受体：通过结合或药理学研究及生理功能进行表征。
Cell Mol Neurobiol. 1990 Sep;10(3):303-25. doi: 10.1007/BF00711177.

本文引用的文献

1
Retinal indoleamine accumulating neurons.
Neurochem Int. 1980;1C:209-29. doi: 10.1016/0197-0186(80)90062-5.
2
GABA and dopamine receptor binding in retinal synaptosomal fractions.
Neurochem Int. 1980;1C:167-81. doi: 10.1016/0197-0186(80)90059-5.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Identification of dopamine receptors in the rat retina.大鼠视网膜中多巴胺受体的鉴定。
Exp Eye Res. 1980 Apr;30(4):431-7. doi: 10.1016/0014-4835(80)90058-5.
5
Specificity of serotonin uptake by bovine retina: comparison with tryptamine.牛视网膜对血清素摄取的特异性：与色胺的比较。
Exp Eye Res. 1980 Jul;31(1):31-9. doi: 10.1016/0014-4835(80)90088-3.
6
In vitro experiments on the metabolism, uptake and release of 5-hydroxytryptamine in bovine retina.
Brain Res. 1980 Feb 24;184(2):283-97. doi: 10.1016/0006-8993(80)90799-4.
7
Dopamine receptor binding of 3H-ADTN (2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene) regulated by guanyl nucleotides.鸟苷酸对3H-ADTN（2-氨基-6,7-二羟基-1,2,3,4-四氢萘）多巴胺受体结合的调节作用。
Eur J Pharmacol. 1978 Aug 15;50(4):459-61. doi: 10.1016/0014-2999(78)90156-5.
8
3H-Spiroperidol labels serotonin receptors in rat cerebral cortex and hippocampus.3H-螺哌利多标记大鼠大脑皮层和海马体中的血清素受体。
Eur J Pharmacol. 1978 May 15;49(2):201-2. doi: 10.1016/0014-2999(78)90080-8.
9
Uptake and release of [14C]GABA from rabbit retina synaptosomes.[14C]γ-氨基丁酸在兔视网膜突触体中的摄取与释放
Exp Eye Res. 1977 Sep;25(3):265-75. doi: 10.1016/0014-4835(77)90093-8.
10
Similar binding of 3H-ADTN and 3H-apomorphine to calf brain dopamine receptors.3H-ADTN和3H-阿扑吗啡与小牛脑多巴胺受体的相似结合。
Eur J Pharmacol. 1979 Apr 15;55(2):137-42. doi: 10.1016/0014-2999(79)90385-6.

多巴胺激动剂3H-ADTN与牛视网膜膜的结合。

Binding of 3H-ADTN, a dopamine agonist, to membranes of the bovine retina.

作者信息

Osborne N N

出版信息

Cell Mol Neurobiol. 1981 Jun;1(2):167-74. doi: 10.1007/BF00710717.

DOI:10.1007/BF00710717
PMID:7346167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11572874/
Abstract

The binding of 3H-ADTN, a potent dopamine receptor agonist, to crude membrane preparations of bovine retina was studied, using a filtration method to isolate membrane-bound ligand. Specific binding was found to be saturable and occurred at a single binding site with an affinity constant of 7.3 nM. Binding was sodium-independent, slightly enhanced by Triton X-100 treatment, but drastically reduced by both trypsin and sodium laurylsulphate. The binding sites demonstrated a high degree of pharmacological specificity, with dopamine, apomorphine, and epinine being potent displacers of 3H-ADTN. A higher degree of 3H-ADTN binding was associated with subcellular fractions enriched with conventional synaptosomes rather than fractions enriched with photoreceptor synaptosomes.

摘要

利用过滤法分离膜结合配体，研究了强效多巴胺受体激动剂3H-ADTN与牛视网膜粗制膜制剂的结合情况。发现特异性结合具有饱和性，且发生在单一结合位点，亲和常数为7.3 nM。结合不依赖于钠，经Triton X-100处理后略有增强，但经胰蛋白酶和十二烷基硫酸钠处理后则大幅降低。这些结合位点表现出高度的药理学特异性，多巴胺、阿扑吗啡和去甲肾上腺素是3H-ADTN的强效置换剂。3H-ADTN结合程度较高与富含传统突触体的亚细胞组分相关，而非与富含光感受器突触体的组分相关。