Tanaka S, Hata R, Nagai Y
Coll Relat Res. 1981 Sep;1(5):445-52. doi: 10.1016/s0174-173x(81)80028-3.
A two-dimensional mapping method developed for collagen chains has been applied to the CNBr-peptides of rat collagen types I, II, and III and bovine type V (AB) collagen. The method consists of isoelectric focusing in a 4% polyacrylamide gel-6 M urea-2% Pharmalyte with a pH gradient ranging 5 to 10 in the first dimension and SDS-polyacrylamide slab gel (15%) electrophoresis at pH 8.8 in the second dimension. Characteristic peptide maps were obtained for each type of alpha-chain and can be used for identification of isolated collagen chains. Application of this method to an authentic mixture of collagen types and a fraction isolated from rat skin has indicated that the method is sensitive enough to resolve collagen types with a sample as low as 40 micrograms per alpha-chain.
一种为胶原蛋白链开发的二维映射方法已应用于大鼠I型、II型和III型胶原蛋白以及牛V型（AB）胶原蛋白的溴化氰肽段。该方法包括在第一维中于4%聚丙烯酰胺凝胶-6M尿素-2%两性电解质中进行等电聚焦，pH梯度范围为5至10，在第二维中于pH 8.8条件下进行SDS-聚丙烯酰胺平板凝胶（15%）电泳。每种α链类型都获得了特征性肽图，可用于鉴定分离出的胶原蛋白链。将该方法应用于胶原蛋白类型的真实混合物以及从大鼠皮肤分离的组分，结果表明该方法灵敏度足够高，能够以低至每条α链40微克的样品分辨出不同类型的胶原蛋白。
