Kinuta M, Masuoka N, Mizuhara S
Physiol Chem Phys. 1981;13(6):511-5.
Among carbohydrates, D-glucose, D-mannose, D-galactose, and glycogen yielded a previously described fluorescent pigment, reddish in alkaline medium and yellowish in acid, when heated with L-cysteine in hydrochloric acid solution. D-Fructose and some pentoses yielded other patients. Among tissue hydrolysates, liver, small intestine, brain, and spleen were found rich in the cysteine pigment but little or none was produced from skeletal muscle, blood, or urine. Among subfractions of rat liver cells, the microsomal fraction proved the richest source of the pigment in nmol/mg protein, though the total yield from the soluble fraction was comparable to that from the microsomal fraction. In the latter fraction, only ribosomal particles yielded the pigment; smooth membrane did not. The pigment yield from yeast was comparable to that from liver. Yield from Escherichia coli K-12 was about one-forth that from yeast.
在碳水化合物中，D-葡萄糖、D-甘露糖、D-半乳糖和糖原在盐酸溶液中与L-半胱氨酸加热时，会产生一种先前描述的荧光色素，在碱性介质中呈红色，在酸性介质中呈淡黄色。D-果糖和一些戊糖产生了其他产物。在组织水解产物中，发现肝脏、小肠、大脑和脾脏富含半胱氨酸色素，但骨骼肌、血液或尿液中产生的色素很少或没有。在大鼠肝细胞的亚组分中，微粒体组分被证明是每毫克蛋白质中色素最丰富的来源，尽管可溶性组分的总产量与微粒体组分相当。在后一组分中，只有核糖体颗粒产生色素；光滑膜不产生。酵母产生的色素量与肝脏相当。大肠杆菌K-12产生的色素量约为酵母的四分之一。
