Baccetti B, Renieri T, Soldani P
Scan Electron Microsc. 1981;4:151-6.
This paper has been carried out in order to inquire whether some of the best known human malformations can be recognized by scanning electron microscopy. The SEM appearance of "straight tailed", "empty tailed", "short tailed", "round headed", "double", "old" human spermatozoa is described, and related to the inner structure. Straight tails and empty tails are quite evident at SEM. These defects are due to severe axonemal defects, related to dynein and tubulin deficiencies: "9+0", "arm less" and "axoneme less" spermatozoa are included in this category. Short tails and round heads are still more evident defects, due to complete absence of tail structures or acrosome and easily recognizable at SEM. Double spermatozoa are consistently in patients having abnormally high prolactin level and SEM is obviously sufficient for the diagnosis. Finally, spermatozoa in aged individuals show in SEM a peculiar shape due to absence of immature stages, reduced cytoplasm, disordered axoneme. It is concluded that SEM examination is a good tool for the identification of most of the best known human sperm abnormalities.
开展本研究是为了探究一些最常见的人类畸形精子是否可通过扫描电子显微镜识别。本文描述了“直尾”“空尾”“短尾”“圆头”“双头”“老龄”人类精子在扫描电子显微镜下的外观，并将其与内部结构相关联。直尾和空尾在扫描电子显微镜下相当明显。这些缺陷是由于严重的轴丝缺陷，与动力蛋白和微管蛋白缺乏有关：“9+0”“无臂”和“无轴丝”精子属于此类。短尾和圆头是更明显的缺陷，是由于完全没有尾部结构或顶体，在扫描电子显微镜下易于识别。双头精子在催乳素水平异常高的患者中持续存在，扫描电子显微镜显然足以用于诊断。最后，老龄个体的精子在扫描电子显微镜下呈现出特殊形状，这是由于缺乏未成熟阶段、细胞质减少、轴丝紊乱。结论是，扫描电子显微镜检查是识别大多数最常见人类精子异常的良好工具。
