Molinari J L, Yépez L, Tato P, Méndez L
Ann Immunol (Paris). 1981 Jul-Aug;132D(1):25-41.
An immunogenic complex was obtained from Salmonella typhi by the bacterial acetone powder method. This complex induced in mice a high degree of protection against a challenge with the virulent Salmonella. This immunogenic complex was fractionated at least into 19 fractions when chromatographied on a DEAE-cellulose column. By SDS-polyacrylamide gel electrophoresis, 25 protein bands were observed. Eleven DEAE-cellulose fractions were tested in order to know their immunogenicity. Mice were inoculated with 10 micrograms of protein of each fraction. Seven days after, the mice received a booster. Thirty days after the first inoculation, the animals were challenged with S. typhi resuspended in chondroitin-sulphate at 13%, by the intraperitoneal route. Appropriate control mice were included; 30 min before the challenge, mice had been inoculated with 850 microgram of lead acetate by the intravenous route. The immunogenic complex protected 100% of mice; six of its fractions were good immunogens; one of them, the fraction 4, was shown to contain at least 3 proteins by electrophoresis assay. This fraction induced in mice a high degree of protection against the challenge by the virulent Salmonella. Finally, a ribonucleoprotein purified from this fraction was highly immunogenic to mice against the challenge by 10 LD50 of S. typhi (1 LD50 was equivalent to 2 X 10(6) CFU).
通过细菌丙酮粉法从伤寒沙门氏菌中获得了一种免疫原性复合物。这种复合物在小鼠中诱导出对强毒力沙门氏菌攻击的高度保护作用。当在DEAE - 纤维素柱上进行层析时，这种免疫原性复合物至少被分离成19个组分。通过SDS - 聚丙烯酰胺凝胶电泳，观察到25条蛋白带。为了解其免疫原性，对11个DEAE - 纤维素组分进行了测试。给小鼠接种每个组分10微克的蛋白质。7天后，小鼠接受加强免疫。首次接种30天后，通过腹腔途径用悬浮于13%硫酸软骨素中的伤寒沙门氏菌对动物进行攻击。设置了适当的对照小鼠；在攻击前30分钟，通过静脉途径给小鼠接种850微克醋酸铅。该免疫原性复合物保护了100%的小鼠；其6个组分是良好的免疫原；其中一个组分，即组分4，通过电泳分析显示至少含有3种蛋白质。该组分在小鼠中诱导出对强毒力沙门氏菌攻击的高度保护作用。最后，从该组分中纯化的一种核糖核蛋白对小鼠具有高度免疫原性，可抵抗10倍半数致死剂量的伤寒沙门氏菌攻击（1个半数致死剂量相当于2×10⁶CFU）。
