Atkin I, Ornoy A
Metab Bone Dis Relat Res. 1981;3(3):199-207. doi: 10.1016/0221-8747(81)90009-6.
The transplacental effects of cortisone acetate on the skeleton of neonatal mice has been investigated. Pregnant mice were injected intramuscularly with 0.75 mg cortisone acetate daily on days 11-19 of gestation. The mice were allowed to deliver and pups were weighed on days 1, 3, 5, 10, 15, 20 and 30 and compared to controls. Light microscopy of non-decalcified longitudinal sections of bones revealed mineral precipitates throughout the zone of hypertrophic cartilage most prominent on days 1, 3 and 5 and gradually disappearing on days 10-20. Transmission electron microscopy revealed intracellular calcification of maturing and hypertrophic chondrocytes in addition to abnormal calcification of the interstitial substance. Hydroxylapatite crystals were not necessarily associated with matrix vesicles. Scanning electron microscopy of long bones revealed a wide zone of calcified intercartilagenous matrix representing the calcified proliferating and hypertrophic zones. In this area, the calcospherites varied in size (0.5-1.4 mu in diameter) and were found in both longitudinal and transverse septa. No significant differences in the distribution and shape of cartilage matrix vesicles between experimental and control animals was observed. However, the fact that mineral crystals in experimental animals was not necessarily associated with matrix vesicles points to the possibility that this "pathological calcification" may utilize a different mechanism than the normal process through matrix vesicles.
已对醋酸可的松经胎盘对新生小鼠骨骼的影响进行了研究。在妊娠第11 - 19天，给怀孕小鼠每日肌肉注射0.75毫克醋酸可的松。让小鼠分娩，在出生后第1、3、5、10、15、20和30天对幼崽称重，并与对照组进行比较。对骨骼未脱钙纵切片的光学显微镜检查显示，在整个肥大软骨区有矿物质沉淀，在第1、3和5天最为明显，在第10 - 20天逐渐消失。透射电子显微镜检查显示，成熟和肥大软骨细胞内有钙化，此外间质也有异常钙化。羟基磷灰石晶体不一定与基质小泡相关。对长骨的扫描电子显微镜检查显示，有一个宽的钙化软骨间基质区，代表钙化的增殖区和肥大区。在这个区域，钙球大小不一（直径0.5 - 1.4微米），在纵向和横向隔中均有发现。未观察到实验动物和对照动物之间软骨基质小泡的分布和形状有显著差异。然而，实验动物中的矿物质晶体不一定与基质小泡相关这一事实表明，这种“病理性钙化”可能利用了一种不同于通过基质小泡的正常过程的机制。
