用于在电子显微镜下直接观察冷冻水合标本的仪器。
Instrumentation for direct observation of frozen hydrated specimens in the electron microscope.
作者信息
Hutchinson T E, Johnson D E, MacKenzie A P
出版信息
Ultramicroscopy. 1978;3(3):315-24. doi: 10.1016/s0304-3991(78)80041-2.
PMID:734786
Abstract
Instrumentation and techniques are described for the transfer and observation of frozen hydrated specimens in the transmission electron microscope. The transfer is accomplished without the complexity of a vacuum transfer device but also without significant sublimation of specimen ice or frosting. Examples are given of the transfer and observation of thin sections of rapidly frozen muscle and of rapidly frozen thin film preparations of isolated cells.
摘要
本文描述了在透射电子显微镜中转移和观察冷冻水合标本的仪器和技术。转移过程无需真空转移装置的复杂性，也不会出现标本冰的大量升华或结霜现象。文中给出了快速冷冻肌肉薄片以及分离细胞的快速冷冻薄膜制剂的转移和观察示例。
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