Sula L, Sulová J, Janota J
Z Erkr Atmungsorgane. 1981;157(1):3-9.
The cultivation technique of two mycobacteriophages (D-29 and MyF2 P/59) in continuous cultures with a simple synthetic medium is described. The ATCC-607-strain (M. smegmatis) was used as a host strain. The medium was exchanged every 24 hours for 14 days, then the whole cultivation equipment including waste bottle was hermetically closed and preserved in a thermostat for seven years. Every six months about 250 ml of new liquid medium was added into the cultivation container after finishing the passage cultivation. Both mycobacteriophages could be found out still after 6 years in the mixture of the mycobacteriophages preserved in the waste bottle and in the culture container even after seven years. The possibility of using the technique of continuous cultivation of phages for preparing fresh 24 hours old suspensions necessary for phage typing of the mycobacteria is discussed.
描述了两种分枝杆菌噬菌体（D - 29和MyF2 P/59）在简单合成培养基连续培养中的培养技术。使用ATCC - 607菌株（耻垢分枝杆菌）作为宿主菌株。每24小时更换一次培养基，持续14天，然后将包括废液瓶在内的整个培养设备密封，并在恒温箱中保存7年。传代培养结束后，每隔六个月向培养容器中加入约250毫升新的液体培养基。即使在7年后，在废液瓶和培养容器中保存的分枝杆菌噬菌体混合物中，6年后仍能检测到这两种分枝杆菌噬菌体。讨论了使用噬菌体连续培养技术制备分枝杆菌噬菌体分型所需的新鲜24小时龄悬浮液的可能性。
