Stager C E, Gangadharam P R
J Med Microbiol. 1978 May;11(2):187-91. doi: 10.1099/00222615-11-2-187.
The value of RVA, N-1, 7H10, 7H11 and Sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse Mycobacterium smegmatis strain 607B, and with mycobacteriophage DS6A which lyses Mycobacterium tuberculosis strain H37Rv. The methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. It was found that N-1, 7H10 and 7H11 media gave better overall results than RVA medium for M. smegmatis strain 607B and its phages, and that RVA medium was generally the most useful for M. tuberculositems employed.
评估了用于分枝杆菌噬菌体感染和分枝杆菌裂解研究的RVA、N-1、7H10、7H11和索顿培养基的价值。使用了BGI、BKI、CRI-3、G37和LG等分枝杆菌噬菌体进行实验，所有这些噬菌体都能裂解耻垢分枝杆菌607B菌株，还使用了能裂解结核分枝杆菌H37Rv菌株的DS6A分枝杆菌噬菌体。所采用的方法包括“直接裂解”、“常规试验稀释度”的测量以及噬菌斑形成单位的计数。结果发现，对于耻垢分枝杆菌607B菌株及其噬菌体而言，N-1、7H10和7H11培养基总体上比RVA培养基效果更好；而RVA培养基通常对所使用的结核分枝杆菌最为有用。
