Komar A
Probl Med Wieku Rozwoj. 1981;10:215-22.
The possibility of fertilization and development of polyspermia was studied in mouse oocytes stimulated to development with thermal shock. Fertilization was done using non-incubated and incubated (capacitated) spermatozoa. The experiment was carried out in several versions. It was observed that thermal shock caused different types of oocyte stimulation. The type of stimulation played no role in the process of spermatozoa penetration into the oocytes. The capacitated spermatozoa had a greater ability of penetration into the stimulated oocytes than non-capacitated ones. The possibility of spermatozoa penetration into stimulated oocytes persisted up to 3 hours after stimulation, but they were not transformed into male pronucleus. The causes and mechanism of this observation are discussed. In the conclusion refertilization as the mechanism of dispermic triploidy has been ruled out.
研究了热休克刺激小鼠卵母细胞发育时多精受精及发育的可能性。使用未孵育和已孵育(获能)的精子进行受精。实验分几个版本进行。观察到热休克会引起不同类型的卵母细胞刺激。刺激类型在精子穿透卵母细胞的过程中不起作用。与未获能精子相比,获能精子穿透受刺激卵母细胞的能力更强。精子穿透受刺激卵母细胞的可能性在刺激后长达3小时内仍然存在,但它们不会转化为雄原核。讨论了这一观察结果的原因和机制。结论是排除了再次受精作为双精三倍体机制的可能性。