Colas G
Reprod Nutr Dev (1980). 1981;21(3):399-407.
Six adult Ile-de-France rams of different origins, registered in a Flock book, were used in AI for three consecutive years on the same flock (IF) in March-April and in September. The animals (males and females) were always kept in a sheep-fold (natural daylight conditions). They received the same feed throughout the whole experiment. Using the unselected ejaculate(s) of each ram (1 dose diluted sperm/female), artificial inseminations were carried out after oestrus synchronization (FGA + PMSG). Massal motility, percentage of nigrosin-eosin-stained spermatozoa and percentage of morphologically abnormal sperm were determined (150 cells/smear) on each ejaculate (or pooled ejaculates of each ram) used for insemination. 1.--In the spring, sperm fertility was strongly diminished (47.1 vs 68.4 p. 100 LR; P less than 0.01, respectively, in spring and autumn), and the differences between the rams were significant (31.7 to 70.3 p. 100; 0.01 less than P less than 0.02 in spring vs 60.0 to 80.0; P greater than 0.05 in autumn). 2.--Fertilizing ability was related neither to massal motility nor to the proportion of stained cells, although, on the whole, the latter was somewhat higher in spring than in autumn. In the spring, there was a close relationship between the fertility of the ejaculate and its proportion of abnormalities (r = -0.83; P less than 0.01). Fertility was also related to the percentage of abnormal heads (r = -0.59; P less than 0.05). In autumn, when the proportion of total abnormalities was much lower and almost exclusively made up of slight tail damages, this relationship disappeared. Morphological examination of the semen is thus a good fertility test. These results show the importance of the ram in successful breeding in an anoestrous period. Morphological test in the spring must be used to detect those males which are most affected by increasing daylight since, at that time (March-April), a ram maintains nearly the same morphological profile from one year to the next (Colas, 1980).
选用了6只不同来源、登记在种羊登记簿上的法兰西岛成年公羊，于3月至4月以及9月在同一羊群（IF）中连续三年进行人工授精。这些动物（雄性和雌性）一直饲养在羊圈中（自然光照条件）。在整个实验过程中，它们食用相同的饲料。使用每只公羊未经过筛选的射精精液（1剂稀释精液/母羊），在发情同步处理（FGA + PMSG）后进行人工授精。对用于授精的每份射精精液（或每只公羊的混合射精精液）测定总体活力、伊红 - 黑色素染色精子的百分比以及形态异常精子的百分比（150个细胞/涂片）。1. 在春季，精子受精能力显著下降（春季为47.1%，秋季为68.4%；每100个LR，春季和秋季的P值分别小于0.01），公羊之间的差异显著（春季为31.7%至70.3%；0.01 < P < 0.02，秋季为60.0%至80.0%；P > 0.05）。2. 受精能力既与总体活力无关，也与染色细胞的比例无关，尽管总体而言，后者在春季略高于秋季。在春季，射精精液的受精能力与其异常比例之间存在密切关系（r = -0.83；P < 0.01）。受精能力还与异常头部的百分比有关（r = -0.59；P < 0.05）。在秋季，当总异常比例低得多且几乎全部由轻微的尾部损伤组成时，这种关系消失。因此，精液的形态学检查是一种很好的受精能力测试。这些结果表明公羊在非发情期成功繁殖中的重要性。春季的形态学测试必须用于检测那些受日照增加影响最大的雄性，因为在那个时候（3月至4月），一只公羊从一年到下一年保持几乎相同的形态特征（科拉斯，1980年）。
