Miletić V D, Saracević M, Otasević D
Bilt Hematol Transfuz. 1981;9(1-3):45-52.
Non-agglutinating character of some anti-rabbit erythrocytes (RaRBC) antibodies was demonstrated. Non-agglutinating ("incomplete") antibodies were identified mainly as IgG but in some sera were detected also in IgA and IgD class using indirect Coombs test with monospecific antisera. Neutralization and separation of "complete" (IgM) antibodies were performed using 2-mercaptoethanol treatment and/or preparative immunochemical techniques. Some kind of restriction to kappa light chains and to IgG2 subclass has also been observed but monoclonal character of "incomplete" antibody was excluded. Specificity of reaction of IgG anti-RaRBC antibodies with RaRBC membrane antigens was documented using multiple adsorption-identification experiments. Non-specific (Fc-mediated) reaction was excluded in contrast to previously published results on the presence of Fc receptor on RaRBC.
已证实某些抗兔红细胞（RaRBC）抗体的非凝集特性。非凝集性（“不完全”）抗体主要鉴定为IgG，但在一些血清中，使用单特异性抗血清的间接库姆斯试验也检测到了IgA和IgD类抗体。使用2-巯基乙醇处理和/或制备性免疫化学技术对“完全”（IgM）抗体进行中和与分离。还观察到对κ轻链和IgG2亚类的某种限制，但排除了“不完全”抗体的单克隆特性。通过多次吸附-鉴定实验记录了IgG抗RaRBC抗体与RaRBC膜抗原反应的特异性。与先前发表的关于RaRBC上存在Fc受体的结果相反，排除了非特异性（Fc介导）反应。
