Miletić V D, Saracević M, Otasević D
Bilt Hematol Transfuz. 1980;8(1):45-56.
Agglutinins against rabbit erythrocytes (RaRBC) were detected in 60 investigated normal human ser in titres ranging 1:20 - 1:1280. Titres of saline anti-RaRBC were not dependent to temperature nor to the blood groups of donor sera. Saline agglutinins were identified as antibodies of IgM class based on the result of gelfiltration and absorptions on anion exchanger and SpA-Sepharose immunoadsorbent as well as on mercaptoethanol and sulfitolysis sensitivity. IgG anti-RaRBC antibodies incapable to agglutinate RaRBC in saline were detected using indirect Coombs test in the second protein peak of gelfiltration. Some characteristics of anti-RaRBC saline agglutinins were compared with human natural isohemagglutinins.
在60份被调查的正常人类血清中检测到了抗兔红细胞（RaRBC）凝集素，其效价范围为1:20至1:1280。盐水抗RaRBC的效价既不依赖于温度，也不依赖于供体血清的血型。基于凝胶过滤、阴离子交换剂和葡萄球菌A蛋白-琼脂糖免疫吸附剂上的吸附结果以及对巯基乙醇和亚硫酸盐裂解的敏感性，盐水凝集素被鉴定为IgM类抗体。使用间接库姆斯试验在凝胶过滤的第二个蛋白峰中检测到了在盐水中不能凝集RaRBC的IgG抗RaRBC抗体。将抗RaRBC盐水凝集素的一些特性与人类天然同种血凝素进行了比较。
