Ruzicka T, Goerz G, Vizethum W, Kratka J
Dermatologica. 1980;160(2):135-41. doi: 10.1159/000250487.
Both single intravenous and repeated intracutaneous injections of bacillus Calmette-Guérin (BCG) resulted in alteration of the hepatic microsomal drug-metabolizing enzymes of the rat liver. The cytochrome P-450 content was not significantly altered; its activity (ethoxycoumarin O-dealkylation) was inhibited in both the intravenous and intracutaneous group. The arylhydrocarbon-hydroxylase and aminopyrine-demethylase activities were also diminished; decrease of cytochrome-c-reductase activity was noted after intravenous application only. Comparison of the results for the intravenous and intracutaneous application routes respectively showed qualitative and quantitative differences. The inhibitory effects of BCG treatment on the drug-metabolizing enzymes of the rat liver can only partly be explained by changes in the cytochrome P-450 system. These findings might lead to reconsideration of dosage of drugs in cancer (malignant melanoma) patients treated by combined chemoimmunotherapy.
单次静脉注射和多次皮内注射卡介苗（BCG）均导致大鼠肝脏微粒体药物代谢酶发生改变。细胞色素P-450含量无显著变化；其活性（乙氧香豆素O-脱烷基作用）在静脉注射组和皮内注射组均受到抑制。芳烃羟化酶和氨基比林脱甲基酶活性也降低；仅在静脉注射后观察到细胞色素c还原酶活性下降。分别比较静脉注射和皮内注射途径的结果显示出定性和定量差异。卡介苗治疗对大鼠肝脏药物代谢酶的抑制作用只能部分地通过细胞色素P-450系统的变化来解释。这些发现可能会促使人们重新考虑联合化疗免疫疗法治疗癌症（恶性黑色素瘤）患者时药物的剂量。
