Ramaswamy K, Peterson M A, Flint P W, Whalen G E
Gastroenterology. 1980 Mar;78(3):464-9.
Small intestinal absorptive function was investigated in genetically diabetic mouse model (C57 BL/KSJ dbm) in order to determine the long-term effects of genetic and uncontrolled diabetes mellitus on intestinal function. Initial rates of uptake of nonmetabolizable glucose analogs, beta-methyl-D-glucoside and 3-O-methyl-D-glucose were determined in diabetic mice and their littermate controls using everted sacs from proximal and distal halves of the intestine. In addition, intestinal weight, intestinal length, mucosal protein, and DNA were measured. There were no significant differences between controls and diabetics in rates of uptake by either proximal or distal segments. Kinetic characteristics of uptake, Km and Vmax, were similar in controls and diabetics. These results clearly demonstrate that intestinal transport of monosaccharides is not altered in genetic diabetes, and therefore are in contrast to augmented transport reported in the early phase of drug-induced diabetes but similar to the results observed in chronic drug-induced diabetes. However, diabetic mice exhibited stimulated intestinal growth similar to rats with chronic drug-induced diabetes.
为了确定遗传性和未控制的糖尿病对肠道功能的长期影响，我们在遗传性糖尿病小鼠模型（C57 BL/KSJ dbm）中研究了小肠吸收功能。使用来自小肠近端和远端两半的外翻肠囊，测定了糖尿病小鼠及其同窝对照中不可代谢的葡萄糖类似物β-甲基-D-葡萄糖苷和3-O-甲基-D-葡萄糖的初始摄取率。此外，还测量了肠重量、肠长度、粘膜蛋白和DNA。对照和糖尿病小鼠近端或远端节段的摄取率没有显著差异。对照和糖尿病小鼠摄取的动力学特征Km和Vmax相似。这些结果清楚地表明，遗传性糖尿病中肠道单糖转运没有改变，因此与药物诱导糖尿病早期报道的转运增加形成对比，但与慢性药物诱导糖尿病中观察到的结果相似。然而，糖尿病小鼠表现出与慢性药物诱导糖尿病大鼠相似的肠道生长刺激。
