Frisch E P
Haemostasis. 1980;9(1):1-8. doi: 10.1159/000214334.
The incidence of positive ethanol gelation test (EGT) after addition of brinase (a proteolytic enzyme preparation from Aspergillus oryzae) to anticoagulated and non-anticoagulated human plasma was studied. In vitro addition of brinase to plasma causes positive EGT, and the incidence is dose-dependent. In plasma from warfarin-treated patients and/or after addition of heparin to plasma, prior to the addition of brinase, a significantly reduced incidence of positive EGT is observed. The incidence is lowest after heparin. Gels formed in the presence of heparin are easier susceptible to enzymatic degradation than those formed in the absence of heparin.
研究了在抗凝和未抗凝的人血浆中添加米曲霉蛋白酶（一种来自米曲霉的蛋白水解酶制剂）后乙醇凝胶试验（EGT）呈阳性的发生率。在体外向血浆中添加米曲霉蛋白酶会导致EGT呈阳性，且发生率呈剂量依赖性。在华法林治疗患者的血浆中，和/或在向血浆中添加肝素后，在添加米曲霉蛋白酶之前，观察到EGT呈阳性的发生率显著降低。肝素处理后发生率最低。与未添加肝素时形成的凝胶相比，在肝素存在下形成的凝胶更容易被酶降解。
