经弹性蛋白酶消化溶解的肾小球基底膜抗原的免疫学特性。

Immunological properties of glomerular basement membrane antigens solubilized by elastase digestion.

作者信息

Stuffers-Heiman M, Tjoeng-Mutsaerts N, Ferwerda W, Van Es L A

出版信息

J Immunol Methods. 1980;32(1):93-102. doi: 10.1016/0022-1759(80)90120-9.

Abstract

Glomerular basement membrane (GBM) was solubilized by hog pancreas elastase and fractionated on Bio-Gel P-2. The composition and antigenic properties of elastase-solubilized GBM were compared with those of whole GBM. Elastase-solubilized bovine GBM (ES-BoGBM) and whole bovine GBM (BoGBM) showed similar amino acid and carbohydrate compositions, suggesting that neither the glycoprotein nor the collagen component of the GBM was selectively solubilized. Administration of rabbit antibodies against elastase-solubilized rat GBM (ES-RtGBM) and whole rat GBM (RtGBM) to rats showed that these preparations had similar nephritogenic properties although the kidney saturation dose was higher with antibodies to ES-RtGBM, suggesting that rabbits may recognize antigenic sites in ES-RtGBM which are not exposed in RtGBM. Radiolabeled ES-BoGBM or ES-RtGBM could be used in a radioimmunoassay to measure the heterologous immune response and the autoimmune response in rats immunized with BoGBM.

摘要

肾小球基底膜（GBM）用猪胰弹性蛋白酶溶解，并在生物凝胶P-2上进行分级分离。将弹性蛋白酶溶解的GBM的组成和抗原特性与完整GBM的进行比较。弹性蛋白酶溶解的牛GBM（ES-BoGBM）和完整牛GBM（BoGBM）显示出相似的氨基酸和碳水化合物组成，表明GBM的糖蛋白和胶原蛋白成分均未被选择性溶解。给大鼠注射抗弹性蛋白酶溶解的大鼠GBM（ES-RtGBM）和完整大鼠GBM（RtGBM）的兔抗体，结果显示这些制剂具有相似的致肾炎特性，尽管抗ES-RtGBM抗体的肾脏饱和剂量更高，这表明兔子可能识别ES-RtGBM中未暴露于RtGBM的抗原位点。放射性标记的ES-BoGBM或ES-RtGBM可用于放射免疫测定，以测量用BoGBM免疫的大鼠中的异源免疫反应和自身免疫反应。