Van Der Schueren B, Cassiman J J, Van Den Berghe H
J Invest Dermatol. 1980 Jan;74(1):29-35. doi: 10.1111/1523-1747.ep12514579.
Skin biopsies are routinely used to establish fibroblast cultures in vitro, but the ultrastructural properties of the cells during this outgrowth have not received much attention. In the present study, the rate and the nature of the outgrowth of skin biopsy explants were examined in a quantitative and qualitative fashion. Attention was particularly focused on the morphogenetic properties of the various cell types present in the initial outgrowth. The rate of success in obtaining an outgrowth from the explants was slightly dependent on the composition of the medium or the serum. In more than 95% of the cases an initial epithelial outgrowth was obtained. At a later stage the outgrowth became fibroblastic. Remarkable differences in the behavior and ultrastructural properties of epithelial and fibroblastic cells were observed. Incorporation of 3H-thymidine occurred almost simultaneously with the epithelial outgrowth but preceded the migration of the fibroblasts. Under scanning and transmission electron microscopy, the cells of the outermost layers of the epithelium remained flat and polygonal and were covered with small villi during migration. The cells were closely apposed. The germinative cells had a spiky surface and were separated by large intercellular spaces. No basal lamina was formed by the migrating cells. The fibroblasts maintained their shape and smooth surface and reached the substratum under the epithelial outgrowth. They accumulated microfilaments and microtubules in their cytoplasm and were characterized by surface-associated extracellular material. On the substratum, the fibroblasts spreadout. The behavior and ultrastructural properties of these fibroblasts resemble closely those observed on fibroblasts growing-out from aggregates.
皮肤活检常用于体外建立成纤维细胞培养体系，但在此生长过程中细胞的超微结构特性尚未受到太多关注。在本研究中，以定量和定性方式研究了皮肤活检外植体的生长速率和性质。特别关注了初始生长中存在的各种细胞类型的形态发生特性。从外植体获得生长的成功率在一定程度上取决于培养基或血清的组成。在超过95%的病例中获得了初始上皮生长。在后期，生长变为成纤维细胞样。观察到上皮细胞和成纤维细胞在行为和超微结构特性上有显著差异。3H-胸腺嘧啶核苷的掺入几乎与上皮生长同时发生，但先于成纤维细胞的迁移。在扫描电子显微镜和透射电子显微镜下，上皮最外层的细胞在迁移过程中保持扁平多边形，表面覆盖着小绒毛。细胞紧密相邻。生发细胞表面有刺，被大的细胞间隙分隔。迁移细胞未形成基底层。成纤维细胞保持其形状和平滑表面，并到达上皮生长下方的基质。它们在细胞质中积累微丝和微管，并以表面相关的细胞外物质为特征。在基质上，成纤维细胞铺展。这些成纤维细胞的行为和超微结构特性与从聚集体中生长出的成纤维细胞非常相似。
